Subaqueous 3D stem cell spheroid levitation culture using anti-gravity bioreactor based on sound wave superposition.

ABSTRACT:

Background

Recently, various studies have revealed that 3D cell spheroids have several advantages over 2D cells in stem cell culture. However, conventional 3D spheroid culture methods have some disadvantages and limitations such as time required for spheroid formation and complexity of the experimental process. Here, we used acoustic levitation as cell culture platform to overcome the limitation of conventional 3D culture methods.

Methods

In our anti-gravity bioreactor, continuous standing sonic waves created pressure field for 3D culture of human mesenchymal stem cells (hMSCs). hMSCs were trapped and aggerated in pressure field and consequently formed spheroids. The structure, viability, gene and protein expression of spheroids formed in the anti-gravity bioreactor were ana

SUBMITTER: Park JH 

PROVIDER: S-EPMC10197840 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature