Regenerated silk fibroin based on small aperture scaffolds and marginal sealing hydrogel for osteochondral defect repair.

ABSTRACT:

Background

Osteochondral defects pose an enormous challenge without satisfactory repair strategy to date. In particular, the lateral integration of neo-cartilage into the surrounding native cartilage is a difficult and inadequately addressed problem determining tissue repair's success.

Methods

Regenerated silk fibroin (RSF) based on small aperture scaffolds was prepared with n-butanol innovatively. Then, the rabbit knee chondrocytes and bone mesenchymal stem cells (BMSCs) were cultured on RSF scaffolds, and after induction of chondrogenic differentiation, cell-scaffold complexes strengthened by a 14 wt% RSF solution were prepared for in vivo experiments.

Results

A porous scaffold and an RSF sealant exhibiting biocompatibility and excellent adhesive properties are deve

SUBMITTER: Luo Y 

PROVIDER: S-EPMC10197849 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature