Adhesion analysis via a tumor vasculature-like microfluidic device identifies CD8⁺ T cells with enhanced tumor homing to improve cell therapy.

ABSTRACT: CD8⁺ T cell recruitment to the tumor microenvironment is critical for the success of adoptive cell therapy (ACT). Unfortunately, only a small fraction of transferred cells home to solid tumors. Adhesive ligand-receptor interactions have been implicated in CD8⁺ T cell homing; however, there is a lack of understanding of how CD8⁺ T cells interact with tumor vasculature-expressed adhesive ligands under the influence of hemodynamic flow. Here, the capacity of CD8⁺ T cells to home to melanomas is modeled ex vivo using an engineered microfluidic device that recapitulates the hemodynamic microenvironment of the tumor vasculature. Adoptively transferred CD8⁺ T cells with enhanced adhesion in flow in vitro and tumor homing in vivo improve tumor

SUBMITTER: Camargo CP 

PROVIDER: S-EPMC10197969 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature