Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source.

ABSTRACT: High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complet

SUBMITTER: Gul M 

PROVIDER: S-EPMC10198979 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature