Nicotinamide ameliorates mitochondria-related neuronal apoptosis and cognitive impairment via the NAD⁺/SIRT3 pathway.

ABSTRACT: Emerging evidence suggests that mitochondria play a central role in mental health disorders including schizophrenia. Here we investigated whether nicotinamide (NAM) normalized cognitive impairment via a mechanism involving the mitochondrial Sirtuin 3 (SIRT3) pathway. The 24 h maternal separation (MS) rat model was used to mimic schizophrenia-associate phenotypes. Schizophrenia-like behaviors and memory impairments were detected using the pre-pulse inhibition test, novel object recognition test, and Barnes maze test, and neuronal apoptosis was characterized using multiple assays. SIRT3 activity was inhibited pharmacologically or by knockdown in HT22 cells, and BV2 microglia and SIRT3-knockdown HT22 cells were co-cultured in vitro. Mitochondrial molecules were measured by western blotting, a

SUBMITTER: Hao K 

PROVIDER: S-EPMC10199898 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature