Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis.

ABSTRACT: Compensatory endocytosis keeps the membrane surface area of secretory cells constant following exocytosis. At chemical synapses, clathrin-independent ultrafast endocytosis maintains such homeostasis. This endocytic pathway is temporally and spatially coupled to exocytosis; it initiates within 50 ms at the region immediately next to the active zone where vesicles fuse. However, the coupling mechanism is unknown. Here, we demonstrate that filamentous actin is organized as a ring, surrounding the active zone at mouse hippocampal synapses. Assuming the membrane area conservation is due to this actin ring, our theoretical model suggests that flattening of fused vesicles exerts lateral compression in the plasma membrane, resulting in rapid formation of endocytic pits at the border between the ac

SUBMITTER: Ogunmowo TH 

PROVIDER: S-EPMC10199930 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature