Regulatory role of miR-146a in corneal epithelial wound healing via its inflammatory targets in human diabetic cornea.

ABSTRACT:

Purpose

MiR-146a upregulated in limbus vs. central cornea and in diabetic vs. non-diabetic limbus has emerged as an important immune and inflammatory signaling mediator in corneal epithelial wound healing. Our aim was to investigate the potential inflammation-related miR-146a target genes and their roles in normal and impaired diabetic corneal epithelial wound healing.

Methods

Our previous data from RNA-seq combined with quantitative proteomics of limbal epithelial cells (LECs) transfected with miR-146a mimic vs. mimic control were analyzed. Western blot and immunostaining were used to confirm the expression of miR-146a inflammatory target proteins in LECs and organ-cultured corneas. Luminex assay was performed on conditioned media at 6- and 20-h post-wounding in miR-146a mi

SUBMITTER: Poe AJ 

PROVIDER: S-EPMC10200270 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature