SOX9 Modulates the Transformation of Gastric Stem Cells Through Biased Symmetric Cell Division.

ABSTRACT:

Background & aims

Transformation of stem/progenitor cells has been associated with tumorigenesis in multiple tissues, but stem cells in the stomach have been hard to localize. We therefore aimed to use a combination of several markers to better target oncogenes to gastric stem cells and understand their behavior in the initial stages of gastric tumorigenesis.

Methods

Mouse models of gastric metaplasia and cancer by targeting stem/progenitor cells were generated and analyzed with techniques including reanalysis of single-cell RNA sequencing and immunostaining. Gastric cancer cell organoids were genetically manipulated with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) for functional studies. Cell division was determined

SUBMITTER: Chen Q 

PROVIDER: S-EPMC10200757 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature