Regulation of CD19 CAR-T cell activation based on an engineered downstream transcription factor.

ABSTRACT: CAR-T cells present a highly effective therapeutic option for several malignant diseases, based on their ability to recognize the selected tumor surface marker in an MHC-independent manner. This triggers cell activation and cytokine production, resulting in the killing of the cancerous cell presenting markers recognized by the chimeric antigen receptor. CAR-T cells are highly potent serial killers that may cause serious side effects, so their activity needs to be carefully controlled. Here we designed a system to control the proliferation and activation state of CARs based on downstream NFAT transcription factors, whose activity can be regulated via chemically induced heterodimerization systems. Chemical regulators were used to either transiently trigger engineered T cell proliferation or

SUBMITTER: Lainscek D 

PROVIDER: S-EPMC10200817 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature