Single-cell RNA sequencing of peripheral blood reveals immune cell dysfunction in premature ovarian insufficiency.

ABSTRACT:

Background

Premature ovarian insufficiency (POI) is one of the most common causes of female infertility and the etiology is highly heterogeneous. Most cases are idiopathic and the pathogenesis remains unclear. Previous studies proved that the immune system plays a crucial role in POI. However, the precise role of immune system remains unclear. This study aimed to analyze the characteristics of peripheral blood mononuclear cells (PBMC) from patients with POI by single-cell RNA sequencing (scRNA-seq) and to explore the potential involvement of immune response in idiopathic POI.

Methods

PBMC was collected from three normal subjects and three patients with POI. PBMC was subjected to scRNA-seq to identify cell clusters and differently expressed genes (DEGs). Enrichment analysis a

SUBMITTER: Zhang C 

PROVIDER: S-EPMC10200870 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature