Mature primary human osteocytes in mini organotypic cultures secrete FGF23 and PTH1-34-regulated sclerostin.

ABSTRACT:

Introduction

For decades, functional primary human osteocyte cultures have been crucially needed for understanding their role in bone anabolic processes and in endocrine phosphate regulation via the bone-kidney axis. Mature osteocyte proteins (sclerostin, DMP1, Phex and FGF23) play a key role in various systemic diseases and are targeted by successful bone anabolic drugs (anti-sclerostin antibody and teriparatide (PTH1-34)). However, cell lines available to study osteocytes produce very little sclerostin and low levels of mature osteocyte markers. We have developed a primary human 3D organotypic culture system that replicates the formation of mature osteocytes in bone.

Methods

Primary human osteoblasts were seeded in a fibrinogen / thrombin gel around 3D-printed hanging post

SUBMITTER: Knowles HJ 

PROVIDER: S-EPMC10200954 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature