IMMUNEPOTENT CRP increases intracellular calcium through ER-calcium channels, leading to ROS production and cell death in breast cancer and leukemic cell lines.

ABSTRACT: IMMUNEPOTENT CRP (ICRP) is an immunotherapy that induces cell death in cancer cell lines. However, the molecular mechanisms of death are not completely elucidated. Here, we evaluated the implication of intracellular Ca²⁺ augmentation in the cell death induced by ICRP on T-ALL and breast cancer cell lines. Cell death induction and the molecular characteristics of cell death were evaluated in T-ALL and breast cancer cell lines by assessing autophagosome formation, ROS production, loss of mitochondrial membrane potential, ER stress and intracellular Ca²⁺ levels. We assessed the involvement of extracellular Ca²⁺, and the implication of the ER-receptors, IP₃R and RyR, in the cell death induced by ICRP, by using an extracellular calcium chelator and ph

SUBMITTER: Lorenzo-Anota HY 

PROVIDER: S-EPMC10201010 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature