Molecular mechanisms of regulation by a β‐alanine‐responsive Lrp‐type transcription factor from Acidianus hospitalis

ABSTRACT: Abstract The leucine‐responsive regulatory protein (Lrp) family of transcriptional regulators is widespread among prokaryotes and especially well‐represented in archaea. It harbors members with diverse functional mechanisms and physiological roles, often linked to the regulation of amino acid metabolism. BarR is an Lrp‐type regulator that is conserved in thermoacidophilic Thermoprotei belonging to the order Sulfolobales and is responsive to the non‐proteinogenic amino acid β‐alanine. In this work, we unravel molecular mechanisms of the Acidianus hospitalis BarR homolog, Ah‐BarR. Using a heterologous reporter gene system in Escherichia coli, we demonstrate that Ah‐BarR is a dual‐function transcription regulator that is capable of repressing transcription of its own gene and activating trans

SUBMITTER: Bernauw A 

PROVIDER: S-EPMC10201364 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature