The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.

ABSTRACT: Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with sing

SUBMITTER: Winterhalter C 

PROVIDER: S-EPMC10201434 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature