Optimization of ATAC-seq in wheat seedling roots using INTACT-isolated nuclei.

ABSTRACT:

Background

The genetic information contained in the genome of an organism is organized in genes and regulatory elements that control gene expression. The genomes of multiple plants species have already been sequenced and the gene repertory have been annotated, however, cis-regulatory elements remain less characterized, limiting our understanding of genome functionality. These elements act as open platforms for recruiting both positive- and negative-acting transcription factors, and as such, chromatin accessibility is an important signature for their identification.

Results

In this work we developed a transgenic INTACT [isolation of nuclei tagged in specific cell types] system in tetraploid wheat for nuclei purifications. Then, we combined the INTACT system together with the

SUBMITTER: Debernardi JM 

PROVIDER: S-EPMC10201787 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature