Visualising SARS-CoV-2 infection of the lung in deceased COVID-19 patients.

ABSTRACT:

Background

SARS-CoV-2 is a single-stranded positive-sense RNA virus. Several negative-sense SARS-CoV-2 RNA species, both full-length genomic and subgenomic, are produced transiently during viral replication. Methodologies for rigorously characterising cell tropism and visualising ongoing viral replication at single-cell resolution in histological sections are needed to assess the virological and pathological phenotypes of future SARS-CoV-2 variants. We aimed to provide a robust methodology for examining the human lung, the major target organ of this RNA virus.

Methods

A prospective cohort study took place at the University Hospitals Leuven in Leuven, Belgium. Lung samples were procured postmortem from 22 patients who died from or with COVID-19. Tissue sections were fluoresce

SUBMITTER: Van Slambrouck J 

PROVIDER: S-EPMC10202122 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature