Free Radical-Based Sequencing for Native Top-Down Mass Spectrometry.

ABSTRACT: Native top-down proteomics allows for both proteoform identification and high-order structure characterization for cellular protein complexes. Unfortunately, tandem MS-based fragmentation efficiencies for such targets are low due to an increase in analyte ion mass and the low ion charge states that characterize native MS data. Multiple fragmentation methods can be integrated in order to increase protein complex sequence coverage, but this typically requires use of specialized hardware and software. Free-radical-initiated peptide sequencing (FRIPS) enables access to charge-remote and electron-based fragmentation channels within the context of conventional CID experiments. Here, we optimize FRIPS labeling for native top-down sequencing experiments. Our labeling approach is able to access int

SUBMITTER: Rojas Ramirez C 

PROVIDER: S-EPMC10202123 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature