Cellular mechanisms of oligoclonal vascular smooth muscle cell expansion in cardiovascular disease.

ABSTRACT:

Aims

Quiescent, differentiated adult vascular smooth muscle cells (VSMCs) can be induced to proliferate and switch phenotype. Such plasticity underlies blood vessel homeostasis and contributes to vascular disease development. Oligoclonal VSMC contribution is a hallmark of end-stage vascular disease. Here, we aim to understand cellular mechanisms underpinning generation of this VSMC oligoclonality.

Methods and results

We investigate the dynamics of VSMC clone formation using confocal microscopy and single-cell transcriptomics in VSMC-lineage-traced animal models. We find that activation of medial VSMC proliferation occurs at low frequency after vascular injury and that only a subset of expanding clones migrate, which together drives formation of oligoclonal neointimal lesions

SUBMITTER: Worssam MD 

PROVIDER: S-EPMC10202649 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature