Global analysis of herpes simplex virus type 1 transcription using an oligonucleotide-based DNA microarray.

ABSTRACT: More than 100 transcripts of various abundances and kinetic classes are expressed during phases of productive and latent infections by herpes simplex virus (HSV) type 1. To carry out rapid global analysis of variations in such patterns as a function of perturbation of viral regulatory genes and cell differentiation, we have made DNA microchips containing sets of 75-mer oligonucleotides specific for individual viral transcripts. About half of these are unique for single transcripts, while others function for overlapping ones. We have also included probes for 57 human genes known to be involved in some aspect of stress response. The chips efficiently detect all viral transcripts, and analysis of those abundant under various conditions of infection demonstrates excellent correlation with know

SUBMITTER: Stingley SW 

PROVIDER: S-EPMC102029 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature