Human variation impacting <i>MCOLN2</i> restricts <i>Salmonella</i> Typhi replication by magnesium deprivation.

ABSTRACT: Human genetic diversity can reveal critical factors in host-pathogen interactions. This is especially useful for human-restricted pathogens like Salmonella enterica serovar Typhi (S. Typhi), the cause of typhoid fever. One key defense during bacterial infection is nutritional immunity: host cells attempt to restrict bacterial replication by denying bacteria access to key nutrients or supplying toxic metabolites. Here, a cellular genome-wide association study of intracellular replication by S. Typhi in nearly a thousand cell lines from around the world-and extensive follow-up using intracellular S. Typhi transcriptomics and manipulation of magnesium availability-demonstrates that the divalent cation channel mucolipin-2 (MCOLN2 or TRPML2) restricts S. Typhi

SUBMITTER: Gibbs KD 

PROVIDER: S-EPMC10203047 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature