Selecting optimal support grids for super-resolution cryogenic correlated light and electron microscopy.

ABSTRACT: Cryogenic transmission electron microscopy (cryo-TEM) and super-resolution fluorescence microscopy are two popular and ever improving methods for high-resolution imaging of biological samples. In recent years, the combination of these two techniques into one correlated workflow has gained attention as a promising route towards contextualizing and enriching cryo-TEM imagery. A problem that is often encountered in the combination of these methods is that of light-induced damage to the sample during fluorescence imaging that renders the sample structure unsuitable for TEM imaging. In this paper, we describe how absorption of light by TEM sample support grids leads to sample damage, and we systematically explore the importance of parameters of grid design. We explain how, by changing the grid

SUBMITTER: Last MGF 

PROVIDER: S-EPMC10203124 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature