Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase.

ABSTRACT:

Background

Over expression of Reteplase enzyme has already been studies in the periplasmic space of Escherichia coli (E. coli). However, the role different factors in its expresssin rate remained to be elucidated.

Objectives

Optical cell density (OD), IPTG concentration, and expression time are highly effective in the protein expression rates. Therefore, we aimed to determine the optimum levels of these factors for reteplase expression using response surface methodology (RSM).

Materials and methods

The pET21b plasmid was used to sub-clone the designed reteplase gene. Then, the gene was transformed into E. coli BL21 strain. Induction of expression was done by IPTG and analyzed by the SDS page. experiments were designed using the RMS, while the effe

SUBMITTER: Farzaneh F 

PROVIDER: S-EPMC10203180 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature