Dynamic responses of PA to environmental stimuli imaged by a genetically encoded mobilizable fluorescent sensor.

ABSTRACT: Membrane fluidity, permeability, and surface charges are controlled by phospholipid metabolism and transport. Despite the importance of phosphatidic acid (PA) as a bioactive molecule, the mechanical properties of PA translocation and subcellular accumulation are unknown. Here, we used a mobilizable, highly responsive genetically encoded fluorescent indicator, green fluorescent protein (GFP)-N160_RbohD, to monitor PA dynamics in living cells. The majority of GFP-N160_RbohD accumulated at the plasma membrane and sensitively responded to changes in PA levels. Cellular, pharmacological, and genetic analyses illustrated that both salinity and abscisic acid rapidly enhanced GFP-N160_RbohD fluorescence at the plasma membrane, which mainly depended on hydrolysis of ph

SUBMITTER: Li T 

PROVIDER: S-EPMC10203278 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature