A smart pathogen detector engineered from intracellular hydrogelation of DNA-decorated macrophages.

ABSTRACT: Bacterial infection is a major threat to global public health, which urgently requires useful tools to rapidly analyze pathogens in the early stages of infection. Herein, we develop a smart macrophage (Mø)-based bacteria detector, which can recognize, capture, enrich and detect different bacteria and their secreted exotoxins. We transform the fragile native Møs into robust gelated cell particles (GMøs) using photo-activated crosslinking chemistry, which retains membrane integrity and recognition capacity for different microbes. Meanwhile, these GMøs equipped with magnetic nanoparticles and DNA sensing elements can not only respond to an external magnet for facile bacteria collection, but allow the detection of multiple types of bacteria in a single assay. Additionally, we design a propidiu

SUBMITTER: Gui Y 

PROVIDER: S-EPMC10203291 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature