Selection of biophysically favorable antibody variants using a modified Flp-In CHO mammalian display platform.

ABSTRACT: Mammalian display enables the selection of biophysically favorable antibodies from a large IgG antibody library displayed on the plasma membrane of mammalian cells. We constructed and validated a novel mammalian display platform utilizing the commercially available Flp-In CHO cell line as a starting point. We introduced a single copy of a landing pad for Bxb1 integrase-driven recombinase-mediated cassette exchange into the FRT site of the Flp-In CHO line to facilitate the efficient single-copy integration of an antibody display cassette into the genome of the cell line. We then proceeded to demonstrate the ability of our platform to select biophysically favorable antibodies from a library of 1 × 10⁶ displayed antibodies designed to improve the biophysical properties of bococizum

SUBMITTER: Huhtinen O 

PROVIDER: S-EPMC10203562 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature