Uncovering transcriptomic landscape alterations of CAN-2409 in in vitro and in vivo glioma models.

ABSTRACT:

Rationale

CAN-2409 is a locally delivered oncolytic therapy, which results in vaccination against the injected tumor. CAN-2409 consists of a non-replicating adenovirus armed with the Herpes virus thymidine kinase, which metabolizes ganciclovir into a phosphorylated nucleotide that is incorporated into the tumor cell's genome, thereby inflicting immunogenic cancer cell death. While CAN-2409's immunological impact has been well characterized, its effects on the tumor cells transcriptome remains unknown. We compared the transcriptomic landscape after treatment of glioblastoma models with CAN-2409 in vitro and in vivo to assess how the interplay with the tumor microenvironment influences CAN-2409-mediated transcriptome alterations.

Methods

We performed RNA-Seq with

SUBMITTER: Koch MS 

PROVIDER: S-EPMC10203593 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature