Aprepitant inhibits the development and metastasis of gallbladder cancer via ROS and MAPK activation.

ABSTRACT:

Background

Aprepitant, as a neurokinin-1 receptor (NK-1R) antagonist, originally applied for curing chemotherapy-induced nausea and vomiting, has been reported to have significant antitumor effect on several malignant tumors. However, the effect of aprepitant on gallbladder cancer (GBC) is not clear yet. This study aimed to investigate the anti-tumor activity of aprepitant on GBC and the potential mechanisms.

Methods

The NK-1R expression of gallbladder cancer cells were examined by immunofluorescence. MTT assay, wound healing and transwell migration assay were applied to detect the effect of aprepitant on cell proliferation, migration and invasion. Flow cytometry was used to detect the apoptosis rate. The effects of aprepitant on the expressions of cytokine were examined by

SUBMITTER: Cao X 

PROVIDER: S-EPMC10204192 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature