Bioconjugation of Carbohydrates to Gelatin Sponges Promoting 3D Cell Cultures.

ABSTRACT: Gelatin sponges are widely employed as hemostatic agents, and are gaining increasing interest as 3D scaffolds for tissue engineering. To broaden their possible application in the field of tissue engineering, a straightforward synthetic protocol able to anchor the disaccharides, maltose and lactose, for specific cell interactions was developed. A high conjugation yield was confirmed by ¹H-NMR and FT-IR spectroscopy, and the morphology of the resulting decorated sponges was characterized by SEM. After the crosslinking reaction, the sponges preserve their porous structure as ascertained by SEM. Finally, HepG2 cells cultured on the decorated gelatin sponges show high viability and significant differences in the cellular morphology as a function of the conjugated disaccharide. More s

SUBMITTER: Pepe A 

PROVIDER: S-EPMC10204423 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature