Identification of the Binding Epitope of an Anti-Mouse CCR6 Monoclonal Antibody (C₆Mab-13) Using 1× Alanine Scanning.

ABSTRACT: CC chemokine receptor 6 (CCR6) is one of the members of the G-protein-coupled receptor (GPCR) family that is upregulated in many immune-related cells, such as B lymphocytes, effector and memory T cells, regulatory T cells, and immature dendritic cells. The coordination between CCR6 and its ligand CC motif chemokine ligand 20 (CCL20) is deeply involved in the pathogenesis of various diseases, such as cancer, psoriasis, and autoimmune diseases. Thus, CCR6 is an attractive target for therapy and is being investigated as a diagnostic marker for various diseases. In a previous study, we developed an anti-mouse CCR6 (mCCR6) monoclonal antibody (mAb), C₆Mab-13 (rat IgG₁, kappa), that was applicable for flow cytometry by immunizing a rat with the N-terminal peptide of mCCR6.

SUBMITTER: Tanaka T 

PROVIDER: S-EPMC10204553 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature