P- and E-selectin receptor antagonism prevents human leukocyte adhesion to activated porcine endothelial monolayers and attenuates porcine endothelial damage.

ABSTRACT:

Background

Alongside the need to develop more effective and less toxic immunosuppression, the shortage of human organs available for organ transplantation is one of the major hurdles facing the field. Research into xenotransplantation, as an alternative source of organs, has unveiled formidable challenges. Porcine lungs perfused with human blood rapidly sequester the majority of circulating neutrophils and platelets, which leads to inflammation and organ failure within hours, and is not significantly attenuated by genetic modifications to the pig targeted to diminish antibody binding and complement and coagulation cascade activation.

Methods

Here, we model the interaction of freshly isolated human leukocytes with xenotransplanted vasculature under physiologic flow conditions

SUBMITTER: Laird CT 

PROVIDER: S-EPMC10204697 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature