Accuracy improvement via novel ratiometry design in distance-based microfluidic paper based analytical device: instrument-free point of care testing.

ABSTRACT: Developing accurate, precise, instrument-free, and point-of-need microfluidic paper-based devices is highly significant in clinical diagnosis and biomedical analysis. In the present work, a ratiometric distance-based microfluidic paper-based analytical device (R-DB-μPAD), along with a three-dimensional (3D) multifunctional connector (spacer), was designed to improve the accuracy and detection resolution analyses. Specifically, the novel R-DB-μPAD was used for the accurate and precise detection of ascorbic acid (AA) as a model analyte. In this design, two channels were fabricated as detection zones, with a 3D spacer located between the sampling and detection zones to improve the detection resolution by preventing the reagents mixing from overspreading between these zones. Two probes for AA

SUBMITTER: Al-Jaf SH 

PROVIDER: S-EPMC10204734 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature