Exploiting endocytosis for transfection of mRNA for cytoplasmatic delivery using cationic gold nanoparticles.

ABSTRACT:

Introduction

Gene therapy holds promise to cure various diseases at the fundamental level. For that, efficient carriers are needed for successful gene delivery. Synthetic 'non-viral' vectors, as cationic polymers, are quickly gaining popularity as efficient vectors for transmitting genes. However, they suffer from high toxicity associated with the permeation and poration of the cell membrane. This toxic aspect can be eliminated by nanoconjugation. Still, results suggest that optimising the oligonucleotide complexation, ultimately determined by the size and charge of the nanovector, is not the only barrier to efficient gene delivery.

Methods

We herein develop a comprehensive nanovector catalogue comprising different sizes of Au NPs functionalized with two different cationic m

SUBMITTER: Gusta MF 

PROVIDER: S-EPMC10205015 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature