Ultrasensitive qPCR platform for rapid detection of bacterial contamination of raw biological samples at the point of care.

ABSTRACT: Contamination of cell cultures can result in a significant loss of precious biological material, particularly in long-term processes including amplification of chimeric antigen receptors (CAR)-T cells and differentiation of patient-derived stem cells, for therapeutic purposes. Bacterial contamination can also lead to more complex conditions such as sepsis which can cause morbidity and mortality, despite strict controls and good laboratory/manufacturing practices in the manipulation of complex biological samples such as blood used in autologous and allogeneic stem cells transplantation. The current standard method to identify biological risk is the set-up of microbial cultures, which can be time consuming with the likelihood of wasting large amounts of reagents in the event of contamination

SUBMITTER: Garzarelli V 

PROVIDER: S-EPMC10205631 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature