MBTPS2 acts as a regulator of lipogenesis and cholesterol synthesis through SREBP signalling in prostate cancer.

ABSTRACT:

Background

Prostate cancer is the most common cancer in men in the developed world, with most deaths caused by advanced and metastatic disease which has no curative options. Here, we identified Mbtps2 alteration to be associated with metastatic disease in an unbiased in vivo screen and demonstrated its regulation of fatty acid and cholesterol metabolism.

Methods

The Sleeping Beauty transposon system was used to randomly alter gene expression in the Pten^Null murine prostate. MBTPS2 was knocked down by siRNA in LNCaP, DU145 and PC3 cell lines, which were then phenotypically investigated. RNA-Seq was performed on LNCaP cells lacking MBTPS2, and pathways validated by qPCR. Cholesterol metabolism was investigated by Filipin III staining.

Results

Mbtps2 was ident

SUBMITTER: Tibbo AJ 

PROVIDER: S-EPMC10205813 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature