TMEM16A/F support exocytosis but do not inhibit Notch-mediated goblet cell metaplasia of BCi-NS1.1 human airway epithelium.

ABSTRACT: Cl^- channels such as the Ca²⁺ activated Cl^- channel TMEM16A and the Cl^- permeable phospholipid scramblase TMEM16F may affect the intracellular Cl^- concentration ([Cl^-]_i), which could act as an intracellular signal. Loss of airway expression of TMEM16A induced a massive expansion of the secretory cell population like goblet and club cells, causing differentiation into a secretory airway epithelium. Knockout of the Ca²⁺-activated Cl^- channel TMEM16A or the phospholipid scramblase TMEM16F leads to mucus accumulation in intestinal goblet cells and airway secretory cells. We show that both TMEM16A and TMEM16F support exocytosis and release of exocytic vesicles, respectively. Lack of TMEM16A/F expr

SUBMITTER: Centeio R 

PROVIDER: S-EPMC10206426 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature