The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation.

ABSTRACT: Germinal center (GC) formation and antibody production in lymph node follicles require coordinated interactions between B-cells, T-cells and dendritic cells (DCs), orchestrated by the extracellular matrix-rich reticular fiber (RF) network. We describe a unique laminin 523-containing RF network around and between follicles that associates with PDGFrecβ^highCCL19^lowgp38^low fibroblastic reticular cells (FRC). In the absence of FRC expression of laminin α5 (pdgfrb-cre:Lama5^fl/fl), pre-Tfh-cells, B-cells and DCs are displaced from follicle borders, correlating with fewer Tfh-cells and GC B-cells. Total DCs are not altered in pdgfrb-cre:Lama5^fl/fl mice, but cDC2s, which localize to laminin α5 in RFs at follicle bor

SUBMITTER: Song J 

PROVIDER: S-EPMC10206498 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature