Dry loop-mediated isothermal amplification assay for detection of SARS-CoV-2 from clinical specimens.

ABSTRACT:

Objectives

To establish a point-of-care test for coronavirus disease 2019 (COVID-19), we developed a dry loop-mediated isothermal amplification (LAMP) method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA.

Methods

We carried out reverse transcription (RT)-LAMP using the Loopamp SARS-CoV-2 Detection kit (Eiken Chemical, Tokyo, Japan). The entire mixture, except for the primers, is dried and immobilized inside the tube lid.

Results

To determine the specificity of the kit, 22 viruses associated with respiratory infections, including SARS-CoV-2, were tested. The sensitivity of this assay, determined by either a real-time turbidity assay or colorimetric change of the reaction mixture, as evaluated by the naked eye or under illumination with ultr

SUBMITTER: Higashimoto Y 

PROVIDER: S-EPMC10206895 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature