CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.

ABSTRACT:

Background

Post mortem human brain tissue is an essential resource to study cell types, connectivity as well as subcellular structures down to the molecular setup of the central nervous system especially with respect to the plethora of brain diseases. A key method is immunostaining with fluorescent dyes, which allows high-resolution imaging in three dimensions of multiple structures simultaneously. Although there are large collections of formalin-fixed brains, research is often limited because several conditions arise that complicate the use of human brain tissue for high-resolution fluorescence microscopy.

Results

In this study, we developed a clearing approach for immunofluorescence-based analysis of perfusion- and immersion-fixed post mortem human brain tissue, termed hum

SUBMITTER: Woelfle S 

PROVIDER: S-EPMC10207789 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature