Map-based cloning of the APRR2 gene controlling green stigma in bitter gourd (Momordica charantia).

ABSTRACT: Bitter gourd is an economically important vegetable and medicinal crop distinguished by its bitter fruits. Its stigma color is widely used to assess the distinctiveness, uniformity, and stability of bitter gourd varieties. However, limited researches have been dedicated to genetic basis of its stigma color. In this study, we employed bulked segregant analysis (BSA) sequencing to identify a single dominant locus McSTC1 located on pseudochromosome 6 through genetic mapping of an F₂ population (n =241) derived from the cross between green and yellow stigma parental lines. An F₂-derived F₃ segregation population (n = 847) was further adopted for fine mapping, which delimited the McSTC1 locus to a 13.87 kb region containing one predic

SUBMITTER: Zhan J 

PROVIDER: S-EPMC10208069 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature