Basal body organization and cell geometry during the cell cycle in Tetrahymena thermophila.

ABSTRACT: Tetrahymena thermophila possesses arrays of motile cilia that promote fluid flow for cell motility. These consist of intricately organized basal bodies (BBs) that nucleate and position cilia at the cell cortex. Tetrahymena cell geometry and spatial organization of BBs play important roles in cell size, swimming, feeding, and division. How cell geometry and BB organization are established and maintained remains poorly understood, and prior studies have been limited due to difficulties in accurate BB identification and small sample size. We therefore developed an automated image processing pipeline that segments single cells, distinguishes unique BB populations, assigns BBs into distinct ciliary rows, and distinguishes new from mature BBs. We identified unique features to descr

SUBMITTER: Sun H 

PROVIDER: S-EPMC10208108 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature