A genome-wide association study of freezing tolerance in red clover (Trifolium pratense L.) germplasm of European origin.

ABSTRACT: Improvement of persistency is an important breeding goal in red clover (Trifolium pratense L.). In areas with cold winters, lack of persistency is often due to poor winter survival, of which low freezing tolerance (FT) is an important component. We conducted a genome wide association study (GWAS) to identify loci associated with freezing tolerance in a collection of 393 red clover accessions, mostly of European origin, and performed analyses of linkage disequilibrium and inbreeding. Accessions were genotyped as pools of individuals using genotyping-by-sequencing (pool-GBS), generating both single nucleotide polymorphism (SNP) and haplotype allele frequency data at accession level. Linkage disequilibrium was determined as a squared partial correlation between the allele frequencies o

SUBMITTER: Zanotto S 

PROVIDER: S-EPMC10208120 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature