Characterizing and utilizing oxygen-dependent promoters for efficient dynamic metabolic engineering.

ABSTRACT: Promoters adjust cellular gene expression in response to internal or external signals and are key elements for implementing dynamic metabolic engineering concepts in fermentation processes. One useful signal is the dissolved oxygen content of the culture medium, since production phases often proceed in anaerobic conditions. Although several oxygen-dependent promoters have been described, a comprehensive and comparative study is missing. The goal of this work is to systematically test and characterize 15 promoter candidates that have been previously reported to be induced upon oxygen depletion in Escherichia coli. For this purpose, we developed a microtiter plate-level screening using an algal oxygen-independent flavin-based fluorescent protein and additionally employed flow cytometry analy

SUBMITTER: Wichmann J 

PROVIDER: S-EPMC10208419 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature