TrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation in Drosophila.

ABSTRACT: Adult stem cells are essential for tissue maintenance and repair. Although genetic pathways for controlling adult stem cells are extensively investigated in various tissues, much less is known about how mechanosensing could regulate adult stem cells and tissue growth. Here, we demonstrate that shear stress sensing regulates intestine stem cell proliferation and epithelial cell number in adult Drosophila. Ca²⁺ imaging in ex vivo midguts shows that shear stress, but not other mechanical forces, specifically activates enteroendocrine cells among all epithelial cell types. This activation is mediated by transient receptor potential A1 (TrpA1), a Ca²⁺-permeable channel expressed in enteroendocrine cells. Furthermore, specific disruption of shear stress, but not chem

SUBMITTER: Gong J 

PROVIDER: S-EPMC10208578 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature