Circulating cell-free messenger RNA secretome characterization of primary sclerosing cholangitis.

ABSTRACT:

Background

Primary sclerosing cholangitis (PSC) is a rare chronic cholestatic liver disease characterized by multifocal bile duct strictures. To date, underlying molecular mechanisms of PSC remain unclear, and therapeutic options are limited.

Methods

We performed cell-free messenger RNA (cf-mRNA) sequencing to characterize the circulating transcriptome of PSC and noninvasively investigate potentially bioactive signals that are associated with PSC. Serum cf-mRNA profiles were compared among 50 individuals with PSC, 20 healthy controls, and 235 individuals with NAFLD. Tissue and cell type-of-origin genes that are dysregulated in subjects with PSC were evaluated. Subsequently, diagnostic classifiers were developed using PSC dysregulated cf-mRNA genes.

Results

Differentia

SUBMITTER: Chalasani N 

PROVIDER: S-EPMC10208702 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature