Structural basis of catalytic activation in human splicing.

ABSTRACT: Pre-mRNA splicing follows a pathway driven by ATP-dependent RNA helicases. A crucial event of the splicing pathway is the catalytic activation, which takes place at the transition between the activated B^act and the branching-competent B^* spliceosomes. Catalytic activation occurs through an ATP-dependent remodelling mediated by the helicase PRP2 (also known as DHX16)^1-3. However, because PRP2 is observed only at the periphery of spliceosomes^3-5, its function has remained elusive. Here we show that catalytic activation occurs in two ATP-dependent stages driven by two helicases: PRP2 and Aquarius. The role of Aquarius in splicing has been enigmatic^6,7. Here the inactivation of Aquarius leads to the stalling of a spliceosome intermediate-t

SUBMITTER: Schmitzova J 

PROVIDER: S-EPMC10208982 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature