Topographical influence of electrospun basement membrane mimics on formation of cellular monolayer.

ABSTRACT: Functional unit of many organs like lung, kidney, intestine, and eye have their endothelial and epithelial monolayers physically separated by a specialized extracellular matrix called the basement membrane. The intricate and complex topography of this matrix influences cell function, behavior and overall homeostasis. In vitro barrier function replication of such organs requires mimicking of these native features on an artificial scaffold system. Apart from chemical and mechanical features, the choice of nano-scale topography of the artificial scaffold is integral, however its influence on monolayer barrier formation is unclear. Though studies have reported improved single cell adhesion and proliferation in presence of pores or pitted topology, corresponding influence on confluent monolayer

SUBMITTER: Jain P 

PROVIDER: S-EPMC10209110 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature