Developmental dynamics of chromatin accessibility during post-implantation development of monkey embryos.

ABSTRACT:

Background

Early post-implantation development, especially gastrulation in primates, is accompanied by extensive drastic chromatin reorganization, which remains largely elusive.

Results

To delineate the global chromatin landscape and understand the molecular dynamics during this period, a single-cell assay for transposase accessible chromatin sequencing (scATAC-seq) was applied to in vitro cultured cynomolgus monkey (Macaca fascicularis, hereafter referred to as monkey) embryos to investigate the chromatin status. First, we delineated the cis-regulatory interactions and identified the regulatory networks and critical transcription factors involved in the epiblast (EPI), hypoblast, and trophectoderm/trophoblast (TE) lineage specification. Second, we observed that the chromati

SUBMITTER: Dai X 

PROVIDER: S-EPMC10209733 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature