Identification of Metabolite Interference Is Necessary for Accurate LC-MS Targeted Metabolomics Analysis.

ABSTRACT: Targeted metabolomics has been broadly used for metabolite measurement due to its good quantitative linearity and simple metabolite annotation workflow. However, metabolite interference, the phenomenon where one metabolite generates a peak in another metabolite's MRM setting (Q1/Q3) with a close retention time (RT), may lead to inaccurate metabolite annotation and quantification. Besides isomeric metabolites having the same precursor and product ions that may interfere with each other, we found other metabolite interferences as the result of inadequate mass resolution of triple-quadruple mass spectrometry and in-source fragmentation of metabolite ions. Characterizing the targeted metabolomics data using 334 metabolite standards revealed that about 75% of the metabolites generated measurabl

SUBMITTER: Jia Z 

PROVIDER: S-EPMC10209981 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature