Real-Time Immunosensor for Small-Molecule Monitoring in Industrial Food Processes.

ABSTRACT: Industrial food processes are monitored to ensure that food is being produced with good quality, yield, and productivity. For developing innovative real-time monitoring and control strategies, real-time sensors are needed that can continuously report chemical and biochemical data of the manufacturing process. Here, we describe a generalizable methodology to develop affinity-based biosensors for the continuous monitoring of small molecules in industrial food processes. Phage-display antibody fragments were developed for the measurement of small molecules, as exemplified with the measurement of glycoalkaloids (GAs) in potato fruit juice. The recombinant antibodies were selected for use in a competition-based biosensor with single-molecule resolution, called biosensing by particle motion, usi

SUBMITTER: Vu C 

PROVIDER: S-EPMC10209984 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature