Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective.

ABSTRACT: Cas9 is an RNA-guided endonuclease from the type II CRISPR-Cas system that employs RNA-DNA base pairing to target and cleave foreign DNA in bacteria. Due to its robust and programmable activity, Cas9 has been repurposed as a revolutionary technology for wide-ranging biological and medical applications. A comprehensive understanding of Cas9 mechanisms at the molecular level would aid in its better usage as a genome tool. Over the past few years, single-molecule techniques, such as fluorescence resonance energy transfer, DNA curtains, magnetic tweezers, and optical tweezers, have been extensively applied to characterize the detailed molecular mechanisms of Cas9 proteins. These techniques allow researchers to monitor molecular dynamics and conformational changes, probe essential DNA-protein i

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC10210056 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature